基于SSR标记的玉米品种DNA指纹图谱的构建

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本研究基于SSR标记技术,在初步筛选、确定核心引物并对多重PCR探讨的同时,以玉米杂交种及其亲本为材料,构建一套玉米品种的SSR-DNA指纹图谱。本研究为“中国玉米新品种标准DNA指纹库建立”、玉米品种真实性鉴定和纯度检测提供参考。其研究结果如下: 1.本试验用SSR标记技术对60份玉米自交系进行分析,从160对SSR引物中筛选出62对多态性丰富、稳定性高、重复性好的引物。研究发现,用单一引物不能鉴别所有供试自交系,而用若干引物的组合可区分全部的60份玉米自交系。
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