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Objective:In order to evaluate protective Effect of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Ginseng Radix et Rhizoma hypoxia injury in neonatal rats cardiomyocytes.Method:The primary cultured cardiac muscle cells (6days) were randomly divided into NC group,OGD group,OGD+AG group,OGD +BS group.We established the injury model of primary neonatal rats cardiac muscle cell sugar anoxia / reoxygenation sugar,to survey the effect of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Ginseng Radix et Rhizoma of medicated serum of spontaneous imp frequency,cell viability,superoxde dismutase (SOD),malondialdehyde (MDA),Nitric oxide (NO) and lactate dehydrogenase (LDH) in neonatal rat hypoxia injury cardiomyocytes.Result:Compared with model group,spontaneous imp frequency,cell viability,the activity of SOD were increased obviously,the content of MDA,the secretory volume of NO and the release rate of LDH were decreased obviously (P<0.05) in medication administration groups.After 48h of reoxygenation sugar,Compared with other medicated serum groups,spontaneous imp frequency,the cell viability,the activity of SOD were increased obviously,the content of MDA and the release rate of LDH were decreased obviously (P<0.05) in medicated serum (AG2∶1)group.Conclusion:There was a significant protective effect of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Ginseng Radix et Rhizoma hypoxia injury in neonatal rats cardiomyocytes,the optimization compatibility proportion in AG2∶1.