Soybean is main economic crop in China.Economic loss of soybean product cause by fungous diseases of soybean was huge.Seed-borne internal fungal infected can decrease the rate of seed germination and affect seedling vigor, the infections lead to the low output and bad quantity of soybean.Phomopsis spp.was one of the factors effected the soybean production more.P.longicolla was reported in 2010, it was the first time report in China, P.longicolla cause soybean stem blight was found in Heilongjiang province of China but there was no report about P.longicolla infected soybean seed.For insure the situation of Phomopsis spp.cause the soybean disease in soybean production areas, the research about Phomopsis spp.Such as distribution in soybean production areas, pathogenicity, host range and Variety Resistance were carried out, the result was benefited to Phomopsis spp.Learning, it will be the basis of soybean disease manage and guide the soybean planting.