【摘 要】
：
N2-fixing bacteria belonging to the genus Raoultella of the family Enterobacteriaceae are widely associated with plants.Raoultella sp.strain L03 was isolated from surface-sterilized sugarcane roots.In
【机 构】
：
Sugarcane Research Center,Chinese Academy of Agricultural Sciences;Sugarcane Research Institute,Guan
【出 处】
：
广西甘蔗学会2016年年会暨学术交流会
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N2-fixing bacteria belonging to the genus Raoultella of the family Enterobacteriaceae are widely associated with plants.Raoultella sp.strain L03 was isolated from surface-sterilized sugarcane roots.In this study,we inoculated the strain L03 to microbe-free micropropagated plantlets of the main sugarcane cultivar ROC22 grown in Guangxi,China and determined N2-fixation and association between strain L03 and sugarcane plants.Inoculation of strain L03 increased plant biomass,total N,N concentration and chlorophyll,and relieved N-deficiency symptoms of plants under an N-limiting condition.An 15N isotope dilution assay revealed 15N isotope dilution in the inoculated sugarcane plants and incorporation of the fixed 14N from air into chlorophyll.Moreover,a gfp-tagged and antibiotic-resistant L03 strain was reisolated from surface-sterilized sugarcane plants and was detected in plant tissues by fluorescent microscopy.This study for the first time demonstrates that a Raoultella bacterium is able to fix N2 in association with the plant host.
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