Microalgae biofuel is thought to be one of most potential way of biofuel.However, its industrial application faces the bottlenecks of shortage supply of algal feedstocks and expensive of the production cost.Of which, the cultivation of the microalgae is the most domination.Among the cultivation modes of open pond and photobioreactor(PBR), there are many advantages and disadvantages of each.Generally, cultivation with open pond has been widely used because of its cheap capital investment, easy scale up and small operation difficulty.However, its low biomass productivity and low oil content in feedstocks lead to huge land occupancy, which will seriously limit the feasibility of industrial application of microalgae biofuel.Thus enhance the productivity of microalge cultivation in open pond is significantly important.