【摘 要】
：
目的 寻找先天性心脏病室间隔缺损相关基因-转录因子Pax-8的下游基因.方法 分别提取Pax-8基因敲除小鼠纯合子(Pax-8 KO-/-)和杂合子(Pax-8 KO+/-)的心脏总RNA,利用含31,802个小鼠基因的基因芯片检测两组小鼠基因表达水平,找出差异表达的基因,并经半定量RT-PCR和荧光实时定量PCR技术初步筛选出转录因子Pax-8的下游基因.结果 基因芯片检测发现,Pax-8KO-
【机 构】
：
温州医学院附属第一医院心内科 325000 温州医学院心血管生物和基因研究所 温州医学院实验动物中
【出 处】
：
第五届钱江国际心血管病会议暨2011浙江省心血管病年会
论文部分内容阅读
目的 寻找先天性心脏病室间隔缺损相关基因-转录因子Pax-8的下游基因.方法 分别提取Pax-8基因敲除小鼠纯合子(Pax-8 KO-/-)和杂合子(Pax-8 KO+/-)的心脏总RNA,利用含31,802个小鼠基因的基因芯片检测两组小鼠基因表达水平,找出差异表达的基因,并经半定量RT-PCR和荧光实时定量PCR技术初步筛选出转录因子Pax-8的下游基因.结果 基因芯片检测发现,Pax-8KO-/-组与Pax-8 KO+/-组相比有25个基因表达下调,另有17个基因表达上调.差异基因涉及细胞周期及信号转导的调节因子,直接参与代谢的酶,以及核转录因子等.用半定量RT-PCR验证发现：nuclear receptor subfamily 4,groupA,member 1(NR4A1)基因在Pax-8 KO-/-组上调.定量RT-PCR亦证实在Pax-8 KO-/-组NR4A1基因的表达水平较Pax-8 KO+/-组及Pax-8+/+(野生型)组分别上调1.53倍和4.79倍(P＜0.01).结论 NR4A1基因为转录因子Pax-8的下游基因,可能在先天性心脏病室间隔缺损的发病机制中发挥重要作用.
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