Fruit ripening and senescence are the inimitable process orchestrated by the complex interplays between nutrition,energy and growth factors in response to the developmental and environmental signals.Target of rapamycin(TOR)acts as a master regulator to control cell growth and metabolism by integrating nutrient,energy,and growth factors across eukaryotic organisms.However,little is known about the TOR signaling in fruit ripening and senescence.Here,the active-site TOR inhibitors(asTORis)and rapamycin inducible TOR suppression systems were established to decipher TOR signaling in tomato fruit.Based on this system,we found that asTORis and rapamycin can efficiently inhibit kinase activity of SITOR and SITOR plays a crucial role in promoting the conversion from chloroplast to chromoplast in tomato fruits.Reducing TOR activity through asTORis or rapamycin treatment,the fruit ripening and senescence are significantly accelerated and the chloroplast degenerates into chromoplast in a TOR kinase activity dose dependent manner.Furthermore,the supra-additive effects on ripening and senescence were observed in immature green fruits of tomato when the solution of asTORis+rapamycin was injected into fruits.Microscopic observation of epidermal cell found that more starch granule accumulated in plastids of SITOR suppressing fruits than that of mock treated fruits.The transcriptome profiling revealed that the expression profile of the genes associated with ethylene signaling and carbohydrate metabolism was significantly changed,when TOR was repressed.Fruit metabolome analysis further displayed the reduced carbohydrates and carotenoids,whereas the increasing starch contents was observed in TOR suppressing fruits compared with the control fruits.These results suggest that SITOR plays a vital role in fruit ripening and senescence.