Perilipin 1, a coat protein of lipid droplet, plays a key role in adipocyte lipolysis and fat formation of adipose tissues.However, it is not clear how the expression of perilipinl is affected in the decreased white adipose tissues (WAT) of mice treated with dietary supplement of conjugated linoleic acids (CLA).Here we obtained lipodystrophic mice by dietary administration of CLA which exhibited reduced epididymal (EPI) WAT, aberrant adipocytes and decreased expression of leptin in this tissue.We found both transcription and translation of perilipinl was suppressed significantly in EPI WAT of CLA-treated mice compared to that of control mice.The gene expression of negative regulator tumor necrosis factor c (TNFα) and the positive regulator Peroxisome Proliferator-activated receptor-γ (PPARγ) of perilipinl was up-regulated and down-regulated, respectively.In cultured 3T3-L1 cells the promoter activity ofperilipinl was dramatically inhibited in the presence of CLA.Using ex vivo experiment we found that the basal lipolysis was elevated but the hormone-stimulated lipolysis blunted in adipose explants of CLA-treated mice compared to that of control mice, suggesting that the reduction of perilipinl in white adipose tissues may at least in part contribute to CLA-mediated alternation of lipolysis of WAT.