Nanozyme enhanced immunochromatography assay for the rapid detection of avain influenza virus

来源 :The 9th Asian Biophysics Association Symposium (ABA2015)(第九届 | 被引量 : 0次 | 上传用户：jica330

【摘要】

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【作者】

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Dexi Zhang Demin Duan Kelong Fan Panhe Zhang Wei Liu George Fu Gao xiyun Yan

【机构】

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Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of S

【出处】

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The 9th Asian Biophysics Association Symposium (ABA2015)(第九届

【发表日期】

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2015年9期

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The Fe3O4 magnetic nanoparticles (MNPs) can catalyze the oxidation of peroxidase substrates to make color reactions, which can amplify the detection signal in strip.Using the MNPs instead of colloidal gold as the new biosensor, we developed a novel nanozyme enhanced ICA (nanozyme-ICA) to detect avain influenza virus (AIV).The detection limit was 104 pfu/mL for AIV after the DAB substrate was added.The detection sensitivity of this method was 100-fold higher than standard colloidal gold-ICA and comparable with ELISA, while it is much faster (within 30 minutes) and simpler (without need of specialist facilities).These characteristics make the nanozyme-ICA to be an ideal candidate for the AIV detection in epidemic areas.

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Nanozyme enhanced immunochromatography assay for the rapid detection of avain influenza virus

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