运用CRISPR-Cas9系统在大肠杆菌和塔特姆氏菌中进行多基因编辑

来源 :2015合成生物学青年学者论坛 | 被引量 : 0次 | 上传用户：reefstone

【摘要】

：

　　在构建工业微生物的过程中，我们需要一个能有效进行基因组规模的基因编辑的工具。通过将酿脓链球菌二型CRISPR-Cas9系统运用到大肠杆菌中，我们构建了一个靶向的、连续的多

【作者】

：

蒋宇陈飚段春兰孙兵兵杨俊杰杨晟

【机构】

：

合成生物学重点实验室,植物生理生态研究所,上海生命科学研究院,中国科学院,上海,中国;上海工业生物技术研发中心,上海,中国合成生物学重点实验室,植物生理生态研究所,上海生命科学研究院,中国科学院,上海

【出处】

：

2015合成生物学青年学者论坛

【发表日期】

：

2015年5期

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　　在构建工业微生物的过程中，我们需要一个能有效进行基因组规模的基因编辑的工具。通过将酿脓链球菌二型CRISPR-Cas9系统运用到大肠杆菌中，我们构建了一个靶向的、连续的多基因编辑系统。该系统可对大肠杆菌基因组进行精确修改，包括基因敲除和基因插入，效率高达100％。运用该系统可实现三个基因的同时编辑。另外，该系统在肠杆菌科的另一株菌——塔特姆氏菌中被证实有效，基因敲除的效率可达到100％。

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运用CRISPR-Cas9系统在大肠杆菌和塔特姆氏菌中进行多基因编辑

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