Intronless genes,as a characteristic feature of prokaryotes,are an important resource for the study of the evolution of gene architecture in eukaryotes.In the study,14,623(36.87％)intronless genes in maize were identified and the percentage is greater than that of other poaceae and algae.The number of maize intronless genes on each chromosome has a significant linear correlation with the number of total genes on the chromosome and the length of the chromosomes.