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With the rapid development of mobile communication, radio frequency electromagnetic fields (RF-EMF) has been classified as possible carcinogen to human, however,this conclusion of IARC is based on limited epidemiological findings and lack of solid supports from experimental studies.Especially, there are no consistent data regarding the RF-EMF-induced DNA damage.To systematically address the debate on whether RF-EMF exposure results in DNA damage, here we investigate the effects of 4 W/kg 1800 MHz RF-EMF exposure on DNA damage in wide-type (Atm+/+) and ataxia telangiectasia mutated (Atm-/-) mouse embryonic fibroblasts (MEFs).In Atm+/+ MEFs, we found that RF-EMF exposure can induce DNA single-strand strand breaks (SSBs) at 1 hour of exposure.But the DNA damage were quickly repaired and reduced by activated DNA SSBs repair after prolonged exposure.In Atm-/-MEFs, RF-EMF exposure could induce DNA SSBs and/or double-strand breaks (DSBs) at 12 or 24 hours of exposure, and, like the phenomenon observed in Atm+/+ MEFs, the induced DNA damage were quickly repaired and reduced by the activated DNA SSBs and/or DSBs repair during exposure.In conclusion, under our conditions, 1800 MHz RF-EMF exposure can induce transient DNA damage in Atm+/+ and Atm-/-MEFs, and the DNA damage are quickly repaired and decreased by activated DNA damage repair in cells.