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目的:优化辨体调理膏制剂工艺过程,筛选出合适的煎膏条件,以期为中药膏滋药规范化生产提供依据。方法:采用3因素3水平正交试验法考察膏方药材的煎煮条件(加水量、煎煮次数、煎煮时间),并结合HPLC分析药材有效成分的煎出率和橙皮苷含量,以橙皮苷含量为指标进行方差分析;选用砂锅、陶瓷锅和不锈钢锅进行膏方煎膏容器的筛选,以HPLC分析结果作为筛选标准;采用水加热烊胶、黄酒加热烊胶、水溶微波烊胶考察阿胶、龟甲胶的烊化方法,以紫外分光光谱法测得的蛋白质含量为指标筛选最合适的烊胶方法。结果:选用砂锅为煎膏容器,将膏方药材煎煮3次,每次30 min,加8倍水量,采用传统的黄酒烊胶方式烊化阿胶与龟甲胶,能够保证辨体调理膏有效成分的含量,确保疗效。结论:本方法简便可行、准确可靠,可用于辨体调理膏的制剂工艺过程,也可为其它膏方的制备提供参考。
OBJECTIVE: To optimize the preparation process of different body conditioning creams and screen out the suitable conditions of the ointment to provide the basis for the standardized production of traditional Chinese medicine. Methods: The decocting conditions (adding water, decocting times and decocting time) of the ointment were studied by orthogonal experiment with 3 factors and 3 levels. The decocting rate and hesperidin content of the active ingredients of medicinal materials were analyzed by HPLC Hesperidin content as an indicator of variance analysis; selection of casserole, ceramic pot and stainless steel pot for the screening of ointment ointment container to HPLC analysis as a screening criteria; the use of water to heat glue, yellow wine heating glue, water soluble microwave glue Investigate donkey-hide gelatin, tortoise shell gelification method to UV spectrophotometry measured protein content as an indicator of screening the most suitable method of gel glue. Results: The casserole was selected as the decoction container. The decoction was decocted for 3 times for 30 min each time, with 8 times the amount of water. Traditional gelatin and gelatine gum were used to ensure the effective ingredients Content, to ensure efficacy. Conclusion: The method is simple and feasible, accurate and reliable. It can be used in the preparation process of the body conditioning creams. It can also provide references for the preparation of other creams.