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Objective: The objective is to isolate MAPK1 gene family from Brassica napus, Brassica oleracea and Brassica rapa and analyze the transcriptional expression profiles of MAPK1 in various organs of these three species.Method: On the basis of in silico cloning, the full-length cDNA and gDNA of MAPK1 genes were isolated from the three species using RACE (Rapid Amplification of cDNA Ends) technology.Organ specificity of MAPK1 in various organs of the three species was detected by quantitative real-time PCR (qRT-PCR).