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The hydrolytic reaction of cellulases can occur in the interior of cellulosic fibers,causing tensile strength loss of the fibers.Enlarging the enzyme molecule by enzyme immobilization will limit the hydrolysis more relatively to the surfaces of the fibers.Commercial cellulases were non-covalently immobilized on the reversibly soluble polymers (Eudragit S-1OO and L-1OO).The pH and thermal stabilities of cellulase-Eudragit S-1OO increased in comparison with the free cellulase.Cellulase-Eudragit S-1OO and cellulase-Eudragit L-1OO retained 51% and 42% of their original activities after three cycles of repeated uses,respectively.Moreover,the cotton samples treated with the immobilized cellulases were softer and smoother than untreated samples,and suffered less damage than those treated with the free cellulase.