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To find the protein expression changes in post-harvest maturation of Agaricus bisporus fruitbodies, the technique of iTRAQ-MS/MS (i.e.isobaric tags for relative and absolute quantification coupled two-dimensional liquid chromatography tandem mass spectrometry) was used to analyze the proteoms ofA.bisporus As2796 fruitbodies at Oh, 6h, 12h, 48h after harvesting.