AIM To study the effects of Xuefu Zhuyu Decoction (ZFZYD) on antioxidant and drug metabolism enzymes (DMEs) in liver of rats.METHODS Treatment of rats with 3.51,7.02, and 14.04 g·kg-1 ZFZYD per day for 15 d, microsomes and cytosols isolated from liver tissues were prepared.The activities of several antioxidant enzymes and phase Ⅰ and Ⅱ DMEs were determined.RESULTS The activities of antioxidant enzymes GPx and CAT were not significantly affected by these treatments, SOD were significantly increased following treatment with XFZYD 3.51 and 7.02 g·kg-1 , respectively, XFZYD 3.51 g·kg-1 could significantly induce GST in cytosols.In microsomes, the activities of NADPH-cytochrome P450 reductase, cytochrome b5, CYP 3A, CYP 2E1 and UGT were not affected, but GST were significantly induced with the dosage of 3.51 and 7.02 g·kg-1 and the level of GSH were enhanced in all treatments.CONCLUSION XFZYD could induce the activities of GST and SOD in liver of rats.