Rolling-circle-amplified (RCA) single strand DNAs, thousands of nucleotide(nt)-long, were used as scaffolds to create DNA nanoscale wires with a few short staple strands by following the origami design principle with a turnover at 1.5 turn.The core sequence of the circle template, for generating tens and hundreds of tandemly repeated copies by RCA, was designed according to Seemans sequence design principle for nucleic acid structural engineering.The significance for folding the RCA products into nanoscale shapes lies in the design flexibility of both staple and scaffold strand codes, simplicity of tens or single-digit staple strands to fold periodic template-complement RCA products, and low cost.