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目的:探索鸡矢藤口服液(Paederiae oral liquid,POL)对大鼠Freund’s完全佐剂性关节炎原发及继发病变的作用及作用机制。方法:将大鼠随机分为模型组、雷公藤多苷片组(10.5mg/kg)及POL高(0.563g/kg)、中(0.282 g/kg)、低(0.141 g/kg)剂量组。原发病变实验采用灌胃给POL 3次后足跖注射完全佐剂致炎,于3、6、18 h用游标卡尺测量致炎侧踝关节肿胀度。继发病变实验造模后分组同上,于第15天开始灌胃给予POL,连续14 d,用游标卡尺测量致炎与非致炎侧踝关节肿胀度、评分测定疼痛反应、生化方法测定一氧化氮(NO)和前列腺素E2(PGE2)水平。结果:POL高、中、低剂量能有效抑制原发病变踝关节肿胀度,其中3 h抑制效果最好,抑制率分别为44.52%、31.12%、30.81%(均P<0.01)。POL高、中、低剂量对致炎侧继发病变足肿胀无明显作用,而高剂量组在25 d时对非致炎侧踝关节肿胀度抑制率为42.92%,对非致炎侧的红肿、结节有较强抑制作用,抑制率为68.75%(均P<0.01)。POL高、中、低剂量组对致炎侧炎性组织中NO含量分别降低48.93%、54.07%、48.84%(P<0.05或P<0.01),PGE2含量降低23.56%,18.97%,16.67%。结论:鸡矢藤口服液对大鼠完全佐剂性关节炎原发病变有明显抗炎作用,但对继发病变抗炎作用强度不明显,其作用机制与其有效抑制炎性组织中NO、PGE2含量有关。
Objective: To explore the effect and mechanism of Paederiae oral liquid (POL) on primary and secondary Freund’s adjuvant arthritis in rats. Methods: The rats were randomly divided into three groups: model group, tripterygium glycosides tablet (10.5mg / kg) and POL group (0.563g / kg) . The primary lesion experiment was performed by intragastric injection of complete adjuvant for 3 times after POL injection. The inflammation was induced by an vernier caliper at 3, 6 and 18 hours. After secondary lesion experiment, the rats were divided into the same groups as the above. On the 15th day, POL was given by gavage for 14 consecutive days. The degree of swelling of the ankle joint was measured by vernier caliper. The pain response was measured by the score. The nitric oxide (NO) and prostaglandin E2 (PGE2) levels. RESULTS: High, medium and low doses of POL could effectively inhibit the ankle swelling of primary lesion. Among them, the inhibitory effect was the best at 3 h with the inhibition rates of 44.52%, 31.12% and 30.81%, respectively (all P <0.01). High, medium and low doses of POL had no significant effect on secondary pathological changes of the lesion on the side of inflammation, while the inhibition rate on the non-inflammatory side ankle swelling of the high dose group was 42.92% on the 25th day, , Nodules have a strong inhibitory effect, the inhibition rate was 68.75% (all P <0.01). In the high, middle and low doses of POL group, the content of NO in inflammatory side inflammatory tissues decreased by 48.93%, 54.07%, 48.84%, PGE2 decreased by 23.56%, 18.97%, 16.67%, respectively. CONCLUSION: Chicken rotenone oral liquid has obvious anti-inflammatory effect on the primary adjuvant arthritis in rats, but its anti-inflammatory activity is not obvious to the secondary lesion, its mechanism of action and its effective inhibition of NO, PGE2 content in the inflammatory tissue related.