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Insulin-like growth factor-1 receptor (IGF-1R) is involved in the development of multiple types of cancer and has been intensively investigated. Ample evidence demonstrates that the disruption of IGF-1R signaling could contribute to tumor inhibition. In this study, inhibition of tumor cell proliferation and cell apoptosis were observed in murine melanoma cells treated by inactivated sendai virus (hemagglutinating virus of Japan envelope, HVJ-E), which resulted in the decrease of melanin synthesis and tyrosinase production. Immunoblot, immunohistochemistry and real-time reverse transcription PCR analysis demonstrated that HVJ-E could down-regulate the IGF-1R in murine melanoma cells or transplanted melanomas. Furthermore, retarded transplanted melanoma growth on BALB/c-nude mice and prolonged survival time of the tumor-bearing mice were also observed. HVJ-E mediated down-regulation of IGF-1R expression in melanoma cells may provide new insights for further investigation to discover the potential mechanism in direct tumor killing.