Enamel is the hardest material formed by vertebrates and is the most highly mineralized skeletal tissue present in the body.However,enamel cant heal itself by a cellular repair as enamel is both acellular and avascular.It would loss mineral substance due to caries,trauma and erosion.Restorations of damaged tooth tissues with artificial materials represent the traditional therapeutic solutions.Although many sophisticated materials are now available for restoration,their use is not yet completely satisfactory.However,combination of tissue bioengineering along with the development of genetically designed trigger nanoparticles,which are biomimetic with mineralized tissues,have begun to bear fruit in the manufacturing of in vitro teeth tissue even the whole teeth.This seminar will describe recent research on the nano-hydroxyapatite,as the basic block unit of enamel,shows great potentials in the regeneration of enamels,meanwhile,the challenge and perspective of this non cell-base therapeutic approach in the regenerative dentistry will be also discussed.