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To determine whether herpes simplex virus type-1 (HSV-1) can establish latent infection in tumor cells, two-dimensional (2D) monolayer cultures ofOCM-1 uveal melanoma cells were inoculated with a recombinant HSV-1 strain (K26GFP) that expresses the green fluorescent protein (GFP) upon replication.Cells of the monolayers were scraped off one hour after virus inoculation and were mixed with extracellular matrix (Matrigel)-containing media to establish threedimensional (3D) tumor cultures.We found that some HSV-l-inoculated cells expressed GFP and were destroyed in the 3D cultures.