Cephalochordates, the basal invertebrate chordates within the phylum Chordata, are key to understanding the evolution of developmental mechanisms during the invertebrate-chordate to vertebrate transition.However, one impediment to amphioxus as a model organism for transgenic research and functional assay of cis-regulatory modules (CRMs), such as enhancers and silencers, has the inability to obtain an extremely high efficient molecular tool such as proximal and basal (or core, minimal) promoter.