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Glioma is the most common primary brain malignant tumor.Receptors for activated C-kinases 1 (RACK1) is widely expressed in the central nervous system,and regulates multiple cellular processes including cell survival,proliferation,migration and metastasis.However,the role of RACK1 in glioma has never been revealed.The present study for the first time showed that the RACK1 expression was significantly higher in glioma tissues and cell lines when compared with that in normal brain tissues,and was positively associated with the malignancy of glioma.SiRNA-induced RACK1 downregulation significantly suppressed the proliferation and invasion of human glioma U87 and CHG-5 cells,while promoted their apoptosis via upregulating Bax expression and reducing Bcl-2 expression.Furthermore,forced downregulation of RACK1 notably inhibited tumor xenograft growth in nude mice.These findings suggest that RACK1 play a critical role in the development and progression of glioma in vitro and vivo.Moreover,siRNA-induced RACK1 downregulation remarkably reduced the activity of Src/Akt signaling pathway,which plays an important role in the growth and behavior of human malignancies,indicating that siRNA-mediated RACK1 downregulation inhibited glioma probably via suppressing Src/Akt signaling activity.In conclusion,this study highlights the role of RACK1 in glioma,and makes RACK1 a novel promising therapeutic target for glioma treatment.