Despite the recent advancement of biotechnology and pharmaceutical research,cancers remain the leading cause of human mortality.It is vital to diagnose cancers at an early stage when treatment can dramatically improve prognosis.So far,low-cost and easy to operate devices,which allow efficient isolation and sensitive detection of circulating tumor cells (CTCs) for routine blood screening,remain lacking.This talk will introduce a novel micro fluidic platform which can isolate CTCs from the real blood sample in 30 minutes: this system includes a high throughput blood cell separation chip which can separate white blood cells with CTCs from red blood cells and platelets by inertial and suction actions [1-2].In microfluidic chip the particles suspended in rectangular channels are known to be focused near the inner wall of each cross-section channel as the channel Reynolds number (Re) increases due to the lift force balance and the hydrodynamic interactions of the particles with the wall.The hydrodynamic have two major forces that made the particles migrate.One is the wall repulsion force due to the steric crowding effect between the particle and the wall,and another is the inertial lift force that originates from the shear-gradient of the Poiseuilles flow.The wall repulsion force pushes the particle away from the wall and the inertial lift force draws the particle toward the wall.Hence,the balance between these two oppositely directed forces induces particles or cells equilibrate at a certain position [3].And this talk proposes to employ these two forces together to separate whole blood cells into white and red blood cells in a large volume,as shown in Fig.1.[4].The purified white blood cells/CTCs mixture will be then introduced to interact with a nano structured surface which can allow higher retention rate of CTCs on the surface for sample enrichment by 100 folds from 1/107 upto 1/105 CTCs/WBCs.The enriched sample will go through a final cells self-assembly process into a densed monolayer array on a cell assembly chip for in parallel inspection at high speed [5-6].Traditional cells arrays usually need special structures to trap cells,not only cause more difficulties in operation but also limit the density of tissue culture.In this talk,we propose a simple,rapid and economic technique to form huge quantity of living cells into a self-assembled high density monolayer array for cell screening and tissue engineering with the employment of gravity force and fluid force,as shwon in Fig.2.[6] Different from traditional cells spreading,this novel cells array chip provide a side fluid force pushing cells together with PBS flowing out of the well by evaporation of PBS from the small outlet,thus prventing cells stack into multiple layers.As a result,the CTCs can be identified in 30 minutes by the integration of these three chips altogether.Isolated CTCs will still be in vital and can be further characterized and cultivated for the identification of cancer stem cells for prognosis.