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为探明我国西部草场危害最为严重的5种疯草中内生真菌的种类及其分布,分析疯草内生真菌的遗传规律。本研究对采自青海和内蒙古的小花棘豆、变异黄芪、黄花棘豆、急弯棘豆和甘肃棘豆等疯草样品,分不同部位对疯草内生真菌进行常规分离纯化,结合形态学和分子生物学对所得菌种进行种属鉴定。结果显示,从我国西部草场5种疯草中分离出10种内生真菌,即小双胞腔菌、层出镰刀菌、拟青霉菌、子囊菌、球状茎点霉菌、细链格孢菌、砖红镰刀茵、三线镰刀菌、焦曲霉和褶皱裸孢壳茵,其中镰刀属和青霉属内生真菌为优势菌种,分别占所有菌种分离总数的21.1%和37.3%。结果表明,我国西部疯草中存在多种内生真菌,不同生态环境中的优势菌种存在一定的专一性。
In order to find out the types and distribution of endophytic fungi in the five kinds of mad weeds in western China, the genetic laws of endophytic fungi were analyzed. In this study, the wild endophytic fungi collected from Qinghai and Inner Mongolia, wild astragalus, Oxytropis, Oxytropis, Oxytropis gansu were routinely isolated and purified from different parts of the grass, Molecular biology of the species obtained species identification. The results showed that ten kinds of endophytic fungi were isolated from five kinds of wild grasslands in the west of China. They were Fusarium, Paecilomyces, Ascomycotina, Alternaria, Brick red sickle, Fusarium, Fusarium graminearum, and Fusarium venenatum, in which the endophytic fungi are the dominant species, accounting for 21.1% and 37.3% of the total isolates, respectively. The results showed that there are many kinds of endophytic fungi in the western China, and the dominant species in different ecological environments have certain specificity.