Accurate HPV genotyping is important for clinical follow-up of persistent HPV infection, a recognized tumor promoter in cervical cancer induction, and for evaluation of prevention strategy for the individual patients to be immunized with type-specific HPV vaccines.DNA sequencing is the "gold standard" method for accurate viral genotyping.This speech introduces a robust "low-temperature" PCR system using moderately thermostable, highly processive and proof-reading HiFi(R) DNA polymerases to amplify a conserved HPV L1 gene DNA strand in liquidbased Pap cytology specimens without the commonly required purification steps to prepare nested PCR amplicons for HPV genotyping by direct automated DNA sequencing.