论文部分内容阅读
the number of laticifer cells in bark is one of the most important factors determining the biosynthesis and economic value of rubber trees (Hevea brasiliensis), the differentiation of laticifer cells in planta has been characterized,whereas laticifer-cell differentiation in callus cultures in vitro is largely unknown.In this study, we present molecular and physiological evidences for laticifer-cell differentiation in calli derived from rubber tree anthers.Rt-Pcr analysis showed that three key genes rubber elongation factor (REF), small rubber particle protein (SRPP),and cis-prenyl transferase (CPT) that are essential in latex biosynthesis in rubber tree bark also were transcribed in anther calli, laticifer cell development in callus cultures was age-dependent; the cells began to appear at 58 days after initiation of culture, and the percentage of laticifer cells increased steadily with increasing callus age.