【摘 要】
：
We used a functional genomic approach that integrated simultaneous genomic and transcript microarray, proteomics, and tissue microarray analyses to directly
【机 构】
：
Department of Pathology University of Maryland School of Medicine USA
【出 处】
：
BIT Life Sciences' 1st Annual World Cancer Congess-2008(
论文部分内容阅读
We used a functional genomic approach that integrated simultaneous genomic and transcript microarray, proteomics, and tissue microarray analyses to directly identify putative oncogenes in lung tumorigenesis.We first identified 183 genes with increases in both genomic copy number and transcript in six lung cancer cell lines.Next, we used two-dimensional polyacrylamide gel electrophoresis and mass spectrometry to identify 42 proteins that were overexpressed in the cancer cells relative to normal cells.Comparing the genes with the proteins, we identified four genes-PRDX1, EEF1A2, CALR, and 14-3-3zeta in which elevated protein expression correlated with both increased DNA copy number and increased transcript levels.
其他文献
信贷资金保值增值的障碍与对策陈齐芳（一）近些年来，企业拖欠银行贷款和赖帐现象日益严重，银行信贷资金的保值增值问题越来越引起人们的关注。归纳起来，银行信贷资金难以收回的现象
人们日益深刻地感受到，由于经济体制的变革，社会经济活动中逐渐暴露出了一系列道德上的矛盾，从而引起了人们观念上的分歧。我国传统的计划经济是国家利益一元化的经济体制。在过
迎着初绽的晨曦,合着音乐的节拍,一群白发老人伴歌而舞。歌罢、舞罢,尽兴而归。曲终人散之际,人群里有位人称“顾老师”的顾怀忠老人,猝然倒地,人事不省。幸好市医院急诊室
上、雪山 (中国湎 67X67cm 1988下、水乡回忆 (版画 40x29cm 1983彦涵作品
On the snow-capped mountains (China 湎 67X67cm 1988, Water Memories (print 40x29cm 1983 Ya
The incidence of immunogenicity in clinical trials of humanized and fully-human antibodies is generally low but adverse reactions can still pose a significa
Introduction: Fluorescence in situ hybridization (FISH) is a valuable tool in clinical practice of cancer.However, high false positive and negative rates co
市场经济条件下，企业成本的高低直接影响企业经济效益的好坏。当前煤炭企业经营环境持续好转，煤价有所上涨，但煤炭生产成本也大幅上扬。因此，加强成本管理，降低综合成本，是企业生存
RP215 monoclonal antibody was shown to recognize specifically a carbohydrate-associated epitope of cancer cellderived immunoglobulins designated as CA215.Pr
Genomic abnormalities such as homozygous deletions and loss of heterozygosity and genetic alterations at human chromosome 3p21.3 region are firequently foun
Notch receptor-mediated signaling is an evolutionarily conserved pathway that controls cell fates in diverse developmental processes.Its mis-regulation has