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Cryptosporidium,a worldwide protozoan parasite,is one of the most common causes of diarrhea for humans and animals.The aim of the present study was to determine Cryptosporidium species/genotypes in pre-weaned calves in Shaanxi Province using PCR and sequencing based on small subunit ribosomal RNA(SSU rRNA)gene.A total of 258 fecal samples were collected from pre-weaned calves in 19 different farms of six areas in Shaanxi Province,Northwestern China.Cryptosporidium infection was detected in 14 of 19 farms(73.7%),with the total prevalence of 20.2%(52/258).Both dairy and Qinchuan(beef)cattle were found with Cryptosporidium infection.Three Cryptosporidium species,namely C.bovis(n =26),C.andersoni(n =14)and C.ryanae(n =12),were detected in pre-weaned calves in Shaanxi province,with C.bovis(in 12 of farms)as the most common species in cattle farms.Two additional previously unknown C.ryanae genotypes CRType Ⅲ and Ⅳ were observed in the present study.However,the zoonotic species,C.parvum,was not detected in this study,which suggested a low zoonotic potential in Cryptosporidium infected pre-weaned calves in this province.