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目的探讨白血病干细胞和正常造血干细胞分离方法。方法配制密度为1.062-1.078g/ml、渗透压为310-440mOsm的Nycodenz-NaCl不连续的密度和不连续渗透压梯度的分离液。采集AML患者骨髓5mlEDTA抗凝样本,加入Ficoll-Hypauq密度为1.077g/ml的分离液,分离获得单个核细胞。获得的单个核
Objective To investigate the method of separating leukemic stem cells from normal hematopoietic stem cells. Methods Nycodenz-NaCl discontinuous density and discontinuous osmotic pressure gradient solutions with a density of 1.062-1.078 g / ml and an osmotic pressure of 310-440 mOsm were prepared. AML patients were collected 5mlEDTA anticoagulant sample of bone marrow, Ficoll-Hypauq was added to a density of 1.077g / ml of separation fluid, isolated mononuclear cells. Single kernel obtained