【摘 要】
：
目的：鉴定中华按蚊(Anopheles sinensis)CYP6P5基因并分析其序列特征，为研究中华按蚊CYP6P5基因杀虫剂抗性机制提供理论依据.方法：以催命按蚊(An.funestus)基因CYP6P5为询问基因
【机 构】
：
重庆师范大学生命科学学院,昆虫与分子生物学研究所,重庆401331
【出 处】
：
重庆市昆虫学会2013年学术交流及研讨会
论文部分内容阅读
目的：鉴定中华按蚊(Anopheles sinensis)CYP6P5基因并分析其序列特征，为研究中华按蚊CYP6P5基因杀虫剂抗性机制提供理论依据.方法：以催命按蚊(An.funestus)基因CYP6P5为询问基因,基于中华按蚊转录组测序数据,采用双向Blast方法搜寻中华按蚊CYP6P5 eDNA序列.利用生物信息学分析相关软件对该基因编码蛋白序列的一级结构、疏水区、跨膜区、结构域、3D结构等进行预测分析,并采用最大似然法(ML)建立了蚊虫代表性种的系统发育关系.结果：搜索获得了一条中华按蚊CYP6P5全长cDNA序列.该cDNA序列具备P450超家族和CYP6家族特征性的保守序列,命名为CYP6P5(GeneBank登录号:KF358704).该cDNA全长为1583bp,开放阅读框为1527 bp,编码508个氨基酸,推测其相对分子质量为58.32 kDa,等电点为7.17.分析表明该基因编码的蛋白序列第5～21氨基酸是疏水区,第5～22氨基酸是跨膜区,第36～505氨基酸是P450保守结构域,并具有多个酶活性位点.系统发育关系和相似性分析表明中华按蚊CYP6P5与催命按蚊CYP6P5和冈比亚按蚊(An.gambiae) CYP6P5亲缘关系最近形成一个单系,相似率分别为89.4％和89.0％.结论：为进一步研究中华按蚊CYP6P5基因溴氰菊酯抗性的分子机理奠定了基础。
其他文献
To gain a better understanding of the molecular mechanisms regulating pupal diapause of the onion maggot Delia antiqua,PCR-based suppressive subtractive hyb
Malaria persists today as the most widespread and devastating protozoan disease of humans.Over 3 billion people live under the threat of malaria and it kill
Twenty-three species and two subspecies of the genus Ancistrocerus Wesmael are taxonomically reviewed.Three new record species of this genus from China are
Insect immune responses are precisely regulated to maintain immune balance.Although in locust immune responses and regulatory mechanisms have been well stud
The small heat shock proteins (sHSPs) are a diverse of molecular chaperones with molecular weight ranging from 12 to 43 kDa in different species.It is well
Eumenes asioboreus Kim et Yamane,2001 is newly recorded from Inner Mongolia,China,description and photos are provided,along with a key to the described Chin
6-磷酸海藻糖合成酶(Trehalose-6-phosphate Synthase,TPS)是昆虫海藻糖合成途径中的关键酶,在某些昆虫的滞育过程中起到重要作用.本文根据葱蝇(Delia antiqua)差减杂交文库
Two new species, Ancistrocerus transpunctatus You and Li, sp.n.and Ancistrocerus deqinensis You and Li, sp.n., both from Yunnan Province, China, are describ
通过近年的区系研究，查阅《Zoological Record》以及相关的分类文献,简述了阿蚊属的系统发生地位及分类研究历史和现状；重点分析了阿蚊属各种类在世界动物地理区系和中国动物地
Background: Malaria around China-Myanmar border is a serious health problem in the countries of South-East Asia.An.minimus is a principle malaria vector wit