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Eighty-four multiresistant K.pneumoniae (MDR-KP) isolates from a Chinese hospital from January to October 2012 were evaluated to characterize the co-existence of 16S rRNA methylase and extended-spectrum β-lactamase (ESBL) determinants and association with mobile genetic elements.Among the 83 MDR-KP isolates studied.19 isolates of amikicin-resistant MDR-KP were identified.They carried nineteen armA genes (22.9%) and three rmtB genes (3.6%) and were genetically diverse.CTX-M genes were found in all of the isolates.Among these amrA or rmtB/CTX-M-producing K.pneumoniae isolates, 31.6% carried the carbapenemase genes [blaNPC-2 (26.3%), blaIMP-4(10.5%), blaND.M-1(5.3%)] which made them resistant to imipenem (MIC ≥ 16 μ g/mL).All positie strains possessed qnr-like genes (16 qnrA1, 10 qnrS1 and 7 qnrB4 genes) and eighteen harboured a aac(6)-Ib-cr gene.Mobile elements ISEcp1, IS26, ISCR1, ISAba125 and sul-1 integrons were detected in 19/19 (100%), 16/19 (84.2%), 18/19 (94.7%), 9/19(47.4%), and 18/19 (94.7%) isolates, respectively.The mobilizing elements occurred in different combinations in the study isolates.Majority armA and qnr genes were in MDR-KP strains carrying integrons containing the ISCR1.Close to 80% ofblaFEM-1 and blasHV-12 were linked to I526 while ≥ 90% of blacTX-M, and blaCMYs were linked to ISEcp1.ISAba125 was located upstream of blaNDM-1 and some blaCMY-2 gene.In addition, seven transconjugants were available for fu rther analysis, and armA, qnrS1, acc(6)-Ib-cr, blaCTλ-M-15, blaTEM-1 and blaNDM-1 were all identified on these self-transferable IncA/C plasmids.The ERIC-PCR patterns and MLST types of the isolates were different from each other.This study points to the dissemination of 16S rRNA methylase genes and the prevalence of selected elements implicated in evolution of resistance determinants in collection of clinical K.pneumoniae in China.