【摘 要】
：
目的：探讨人骨形态发生蛋白9(Bone morphogenetic proteins 9,BMP9)在体内外诱导人脐带间充质干细胞成骨分化的作用研究.方法：设立Ad-BMP9处理组或Ad-GFP对照组感染hUC-MSC
【机 构】
：
重庆医科大学附属儿童医院儿科研究所,儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,重庆市干细胞治疗工程技术研究中心重庆医科大学附属儿童医院骨科,重庆,400014
【出 处】
：
中国细胞生物学学会2015年全国学术大会
论文部分内容阅读
目的：探讨人骨形态发生蛋白9(Bone morphogenetic proteins 9,BMP9)在体内外诱导人脐带间充质干细胞成骨分化的作用研究.方法：设立Ad-BMP9处理组或Ad-GFP对照组感染hUC-MSCs,两组细胞分别于3、5、7天进行ALP活性检测,14d后采用免疫组化染色检测骨钙素(Osteocalcin,OCN)、骨桥蛋白(Osteopotin,OPN)的表达情况,21d后茜素红染色检测矿化结节的形成；然后收集不同分组感染后的hUC-MSCs用于裸鼠皮下注射成骨模型的建立,4周后取出离体骨进行MicroCT扫描和分析,并进行H&E,Masson Trichrome、AlcainBlue染色.结果：BMP9处理组的ALP活性、ALP染色和矿化结节形成明显高于对照组,免疫组化染色结果显示BMP9诱导组的OCN、OPG的阳性表达明显高于对照组；裸鼠皮下注射成骨模型的观察结果显示,空白对照组没有形成肉眼可见的皮下包块,仅感染Ad-BMP9的hUC-MSCs能生成异位骨,且形成的异位骨骨量明显,骨密度平均值为396.05±0.60；H&E染色结果显示BMP9诱导生成的异位骨中形成部分成熟的骨基质和骨小梁,Masson Trichrome染色结果显示BMP9明显诱导hUC-MSCs的基质矿化作用,Alcain Blue染色结果显示BMP9明显诱导hUC-MSCs的软骨内成骨作用；结论：BMP9成功诱导人脐带间充质干细胞的体内外成骨作用,为临床骨组织工程的细胞疗法提供了明确的可行性.
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