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Acyl-acyl cartier protein (ACP) thioesterases are enzymes that terminate the intraplastidial fatty acid synthesis in plants by hydrolyzing the acyl-ACP intermediates and releaseing free fatty acids to be incorporated into glycerolipid in the endoplasmic reticulum (ER).These enzymes are classified in two families, FatA and FatB, which differ in amino acid sequence and substrate specificity.We previously isolated and identified both FatA and FatB from a high palmitoleic acid accumulator plant, indicating that FatB has a high preference towards palmitoyl-ACP and selectively transport acyl-ACP 16:0 from plastid to cytosol.