Human gut flora is a complex microbial ecosystem that plays a major role in human health. Under normal conditions, the gut microflora exists in a state of equilibrium with the host that is mutuallybeneficial to the degree that it has been described as a separate "organ" adapted to human physiology.Therefore, keeping the equilibrium of the intestinal flora is one of many important factors affecting humanhealth. Liver injury often causes disruption of the gut microflora through the gut-liver axis, altered bilesecretion and impaired peristaisis. The work addressed two primary issues regarding the relationshipbetween gut microflora and hepatic damage：first, whether or not changes in gut microflora exacerbatedliver damage in a rat model of acute liver injury induced by D-galactosamine (Ga1N) and, second,defining the possible mechanism (s) involved, To achieve this aim, weinduced changes in gut microflorathrough oral supplementation using several different strains of nonpathogenic E. coli, S. enteritidis,probiotics or Gentamicin, Alterations of the intestinal microflora, mucosa, as well as bacterial translocation (BT)，the production of plasma endotoxin levels, and serum IL-10, IL-6, TNF-α, and IL-12concentrations were examined, CD3+ T cells and CD4+ T cells in peripheral blood and Peyers patches inrats were also evaluated.