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目的:卵母细胞透明带厚度(ZT)和双折射值(ZB)的相关性。方法:回顾性分析了214个IVF周期中不同年龄病人、不同成熟度的卵母细胞ZB和ZT。结果:20～29岁组ZT和ZB显著高于≥40岁组(18.24±0.16μm vs.16.67±0.41μm;8.77±0.42 vs.5.25±1.72)(P<0.01),也高于30～39岁组(18.24±0.16μm vs.17.58±0.15μm)(P<0.01),(8.77±0.42 vs.7.59±0.52)(P<0.05);ZT和ZB与年龄均呈负相关,分别为rZT=-0.195和rZB=-0.200(P<0.05),ZT和ZB之间呈正相关r=0.255(P<0.01);MII期卵母细胞ZB、ZT值均显著低于GV期(7.91±0.65 vs.20.77±1.88,17.80±0.22μm vs.19.42±0.43μm)(P<0.01),也低于MI期卵母细胞(7.91±0.65 vs.10.99±1.35;17.80±0.22μm vs.19.03±0.63μm)(P<0.05)。MII期和MI期卵母细胞ZT与ZB呈正相关,相关系数分别为r=0.125和r=0.239(P<0.05),但GV期卵母细胞ZT和ZB之间无明显相关性。结论:卵母细胞ZT和ZB均随病人年龄和成熟度增加而降低,除GV期卵母细胞外,ZT和ZB存在相关关系,ZT能反映病人年龄和成熟度对卵母细胞的影响,ZT在一定程度上可以反映ZB值。
Aims: Correlation of oocyte zona pellucida thickness (ZT) and birefringence (ZB). Methods: ZB and ZT of 214 oocytes of different ages and maturation levels in IVF cycles were retrospectively analyzed. Results: The levels of ZT and ZB in 20 ~ 29 years old group were significantly higher than those in ≥40 years old group (18.24 ± 0.16μm vs.16.67 ± 0.41μm; 8.77 ± 0.42 vs.5.25 ± 1.72) (P <0.01) (P <0.01), (8.77 ± 0.42 vs.7.59 ± 0.52), respectively (P <0.05). There was a negative correlation between ZT and ZB and age (r = -0.195 and rZB = -0.200 respectively (P <0.05). There was a positive correlation between ZT and ZB (r = 0.255, P <0.01). The ZB and ZT values of MII oocytes were significantly lower than those of GV (7.91 ± 0.65 vs. 20.77 ± 1.88,17.80 ± 0.22μm vs.19.42 ± 0.43μm) (P <0.01), and also lower than MI oocytes (7.91 ± 0.65 vs.10.99 ± 1.35; 17.80 ± 0.22μm vs.19.03 ± 0.63μm) (P <0.05). There was a positive correlation between ZT and ZB in oocytes of stage MII and MI (r = 0.125 and r = 0.239, respectively), but there was no significant correlation between ZT and ZB in oocytes of GV stage. CONCLUSIONS: ZT and ZB of oocytes decreased with age and maturity of patients. ZT and ZB correlated with age and maturity of oocytes except GV oocytes. ZT To some extent, can reflect the ZB value.