The evaluation based on the role and contribution of other sub-interaction except for hydrogen bonding interaction in neutral polysaccharide Sol-gel system is the basic scientific question in the carbohydrate chemistry field.The driving and maintaining force of deaccetylated konjac glucomannan in self crimping,aggregating and sol-gel transition is still a matter of controversy in konjac science.In the present study,Pyrene fluorescence probe technology was primary used to test and roughly determinate the presence of hydrophobic interactions.