Metabonomes of mammals contain tens of thousands different types of metabolites which have many different functions with a huge concentration dynamic range,diverse properties and matrices.Quantitative metabonomic analysis is an essential approach for understanding the molecular aspects of mammalian physiology and pathophysiology of various diseases.The combined NMR and MS methods have become increasingly attractive with complementary metabolomic information.In this presentation,we will discuss the requirements of quantitative metabonomics and strategies to fulfill such tasks followed with some recent advances in our lab in sensitive and efficient quantification of metabolites.These include metabolites containing amino groups cover dozens pathways and play vital roles in redox homeostasis and biosyntheses of proteins,nucleotides and neurotransmitters,sterols and bile acids,eicosanoids and acyl carnitines.Such methods will be potentially useful for molecular epidemiological studies of many different biological events.