【摘 要】
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Objective In this study, we examined the influence of Clostridium butyricum on APQ3 expression and the possible role of p38 /JNK signaling pathways in the regulation of AQP3 expression in human colon
【机 构】
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Shanxi Province People's Hospital College of Che
【出 处】
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The 14th Congress of Gastroenterology China(第十四届全国消化系病学术会议)
论文部分内容阅读
Objective In this study, we examined the influence of Clostridium butyricum on APQ3 expression and the possible role of p38 /JNK signaling pathways in the regulation of AQP3 expression in human colon epithelial cells, HT-29 cells.Methods HT-29 cells were treated with supernatant of Clostridium butyricum, The mRNA and protein expression of AQP3 were measured by real-time polymerase chain reaction (RT-PCR) and western blotting, respectively.Activation of p38 and c-Jun N-terminal Kinase (JNK) was evaluated by phosphorylation of P38 and JNK using western blot assay.AQP3 protein expression was determined by western blots after treatment with SB203580, a selective p38 MAPK inhibitor, or SP600125, a selective JNK inhibitor.
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