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目的探讨灯盏花素对Aβ42诱导阿尔茨海默病(AD)大鼠模型学习记忆功能及抗氧化能力的影响。方法采用侧脑室注射Aβ42的方法制备大鼠痴呆模型,将其随机为5组,并设假手术组作为对照组,水迷宫实验测试大鼠学习记忆能力的变化;采用分光光度法测定脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果 Morris水迷宫实验显示,模型组大鼠逃避潜伏期较正常组明显延长,原象限停留时间明显缩短。灯盏花素组大鼠逃避潜伏期较模型组明显缩短,原象限停留时间明显延长。模型组大鼠脑组织GSH-Px、SOD活力下降,MDA含量明显增多;灯盏花素治疗组学习和记忆能力升高,脑组织GSH-Px、SOD活力升高,MDA含量减少,与模型组比较有显著性差异。结论灯盏花素对AD模型大鼠学习记忆障碍具有明显改善作用并且可剂量依赖性的显著改善AD模型大鼠的抗氧化能力。
Objective To investigate the effects of breviscapin on learning and memory function and antioxidant capacity of Aβ42-induced Alzheimer’s disease (AD) rat model. Methods The rat model of dementia was prepared by injecting intracerebroventricular injection of Aβ42, randomly divided into 5 groups. Sham-operation group was used as the control group, water maze test was used to test the learning and memory abilities in rats. Spectrophotometry was used to determine (GSH-Px), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results Morris water maze test showed that the escape latency in model group was significantly longer than that in normal group, and the retention time in original quadrant was significantly shortened. Breviscapine group escape latency was significantly shorter than the model group, the original quadrant of the residence time was significantly longer. The activity of GSH-Px and SOD in brain tissue of model group decreased and the content of MDA increased obviously. The learning and memory abilities of breviscapine treatment group increased, the activity of GSH-Px, SOD increased and the content of MDA decreased in model group There are significant differences. Conclusion Breviscapine has a significant effect on learning and memory impairment in AD model rats and can significantly improve the antioxidant capacity of AD model rats in a dose-dependent manner.