目的 采用北京国源消毒技术研究所提供的高浓度臭氧水生成机,通过实验室试验,观察了蛋白胨对臭氧水杀菌作用的影响,为消毒实际应用提供实验室试验依据。方法 测定臭氧水中加入1％蛋白胨后残留臭氧浓度的变化;以及菌悬液中加入1％蛋白胨后对臭氧水杀菌作用的影响。按照《消毒技术规范（第3版）第一分册试验技术规范》（1999.12）的要求配制含蛋白胨的菌悬液。用碘量法测定臭氧水浓度,杀菌实验采用悬液定量法。结果 研究表明,臭氧水中加入1％蛋白胨后,残留臭氧浓度迅速降低;菌悬液中加入1％蛋白胨后,臭氧水对大肠杆菌的杀灭率显著下降;消毒试验所用臭氧水体积增加到50ml时,作用1min,对大肠杆菌的杀灭率可达99.93％。结论 臭氧水的杀菌作用受蛋白胨影响较大,消毒实际应用时,为保证消毒效果,可通过增加臭氧水的用量来克服有机物的影响。 Objective To observe the effect of peptone on the sterilization of ozonated water through laboratory experiments by using high concentration ozone water generator provided by Beijing Guoyuan Disinfection Technology Institute and to provide experimental basis for the practical application of disinfection. The method was used to determine the change of residual ozone concentration in ozone water after adding 1% peptone, and the effect of 1% peptone on the bactericidal effect of ozone water. Peptone-containing bacterial suspension was prepared according to the requirements of the “Technical Specifications for the Disinfection Technical Specification (Third Edition), Volume 1” (1999.12). Iodometric method was used to determine the concentration of ozone water, bactericidal experiments using suspension quantitative method. Results The results showed that the concentration of residual ozone decreased rapidly when 1% peptone was added into the ozone water. After 1% peptone was added into the bacterial suspension, the killing rate of E. coli by ozone water was significantly decreased. When the volume of ozone water used in disinfection increased to 50ml , The role of 1min, the killing rate of E. coli up to 99.93%. Conclusion The sterilization effect of ozone water is greatly affected by peptone. In the practical application of disinfection, the effect of organic matter can be overcome by increasing the amount of ozone water to ensure the disinfection effect.