Single-molecule experiment can resolve molecular details and dynamic processes which are otherwise smeared in measurements on ensemble or bulk collection of molecules.However,single-molecule fluorescence experiments suffer from feeble signal intensity and usually not strong enough to be detected easily.Therefore,we aim to utilize plasmonic nano-antennas which can create highly enhanced local fields when pumped resonantly,leading to increased Raman scattering and fluorescence intensity.We mainly reported the structure and fabrication process of our gold-nanoantenna array and offered a proper method to conjugate interested proteins to gold nano-structures.Performance of gold nano-antenna coupled with fluorescent proteins is observed through Total Internal Reflection Microscope (TIRFM) system.Through experiments we demonstrated that:i) m-PEG can be used to prevent non-specific binding while fluorescence proteins with Cysteine-tag can bind gold-particle via coordination bond between gold and thiol;ii)pH is an important factor for binding process between gold-layer and fluorescent proteins.