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Haematopoietic necrosis of gibel carp(Carassius auratus gibelio)is caused by cyprinid herpesvirus 2(CyHV-2)and has caused huge economic losses in aquaculture worldwide.Currently the isolation and propagation of CyHV-2 in vitro is very difficult due to the lack of permissive cell lines.Studies on the pathogenesis of CyHV-2 have been hampered because the virus has not been extensively characterized.In this study,a novel cell line from the brain of gibel carp,denoted GiCB,has been established and characterized.Sustainable propagation of CyHV-2 in the GiCB cell line has been confn-med by virus infection and titration,PCR,transmission electron microscopy,immunofluorescence assay and fluorescence in situ hybridization.The GiCB cells showed typical cytopathic effect by day 6 post-infection with CyHV-2 including cell shrinkage,rounding,and cell fusion with cytoplasmic vacuolization.